Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation
نویسندگان
چکیده
منابع مشابه
Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation.
Somatic hypermutation and clonal selection lead to B cells expressing high-affinity antibodies. Here we show that somatic mutations not only play a critical role in antigen binding, they also affect the thermodynamic stability of the antibody molecule. Somatic mutations directly involved in antigen recognition by antibody 93F3, which binds a relatively small hapten, reduce the melting temperatu...
متن کاملRetraction for Wang et al., Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation.
BIOCHEMISTRY Retraction for “Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation,” by Feng Wang, Shiladitya Sen, Yong Zhang, Insha Ahmad, Xueyong Zhu, Ian A. Wilson, Vaughn V. Smider, Thomas J. Magliery, and Peter G. Schultz, which was first published February 25, 2013; 10.1073/ pnas.1301810110 (Proc Natl Acad Sci USA 110:4261–4266). The undersigned autho...
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Using recombinant DNA techniques, we reconstructed a genealogical tree (Sablitzky, F., Wildner, G. & Rajewsky, K. (1985) EMBO J. 4, 345-350) that connects three clonally related B cells producing somatically mutated antibodies to a progenitor cell expressing a germ line-encoded antibody. The somatic mutants had been isolated from an in vivo immune response. The germ line-encoded progenitor anti...
متن کاملThermodynamic and Binding Effects of Antibody Affinity Maturation
S. Sen , F. Wang , S. Sun , T. J. Magliery , P. G. Schultz 4 1 WIL Research Laboratories, LLC, 2 Scripps Research Institute, 3 Ohio State University, 4 The Scripps Research Institute Purpose Affinity maturation involves production of matured antibodies that has increased specific affinity towards an antigen duri the course of an immune response, brought about by B cells. Matured antibodies diff...
متن کاملTrends in antibody sequence changes during the somatic hypermutation process.
Probable germline gene sequences from thousands of aligned mature Ab sequences are inferred using simple computational matching to known V(D)J genes. Comparison of the germline to mature sequences in a structural region-dependent fashion allows insights into the methods that nature uses to mature Abs during the somatic hypermutation process. Four factors determine the residue type mutation patt...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2013
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1301810110